Communication is important for our bodies to function properly, especially at the cellular level. Our bodies utilize enzymes - specific proteins that catalyze chemical reactions - to effectively and quickly communicate many different kinds of messages. PKCdelta is an enzyme that has been implicated in membrane transport, as well as the formation of bile in the liver. Any misregulation of PKCdelta can lead to cholestasis and liver injury. Utilizing a coupled assay, we will determine the enzymatic activity of PKCdelta as it phosphorylates ATP using the UV spectrophotometer. After we have established the standard curve, we will determine the peak enzymatic activity (using the Michaelis-Menten equation) with different variables, such as temperature and enzyme substrate concentration.
Lesley University, Community of Scholars Day, cells, enzymes, protein kinase C, Adenosine triphosphate, proteins, tumors, STEAM
Hassan, Quamrul and Spencer, Madeline, "How Cells Communicate: A Study of PKCdelta Phosphorylation Efficiency" (2016). Community of Scholars Day—Posters. 33.